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Insulin concentrated from acid/ethanol extracts of IM-9 lymphocytes by the use of octadecylsilyl (ODS) silica cartridges averaged 0.13 ng/ml cell volume compared with the 0.12 ng insulin/ml cell culture medium. The insulin content of these cells appears to be less than 1 % of previously reported values and probably could be accounted for by concentration of insulin from the incubation medium on cell receptors.doi:10.2337/diabetes.31.2.182 fatcat:hcpk2r4l6nhllanq3guxssi5aa