Evidence against insulin synthesis BY IM-9 lymphocytes

W. A. Bauman, V. B. Hatcher, M. A. Levitt, R. S. Yalow
1982 Diabetes  
Insulin concentrated from acid/ethanol extracts of IM-9 lymphocytes by the use of octadecylsilyl (ODS) silica cartridges averaged 0.13 ng/ml cell volume compared with the 0.12 ng insulin/ml cell culture medium. The insulin content of these cells appears to be less than 1 % of previously reported values and probably could be accounted for by concentration of insulin from the incubation medium on cell receptors.
doi:10.2337/diabetes.31.2.182 fatcat:hcpk2r4l6nhllanq3guxssi5aa