This study investigated the effect of dietary supplementation with the fermacto prebiotic and Biotronic ® S.E (an acidifier) on the growth performance, carcass characteristics and serum biochemical parameters of broiler chickens. 240 day old Ross 308 broilers were equally distributed into 24 floor pens and reared for 42 days. A basal diet was formulated according to the recommendations of NRC for starter (1 to 21 days) and grower (22 to 42 days) periods and considered as control diet. Four

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... d diets were formulated as listed control diet (without any additives), fermacto prebiotic, Biotronic ® S.E (an acidifier) and mixture of fermacto prebiotic plus Biotronic ® S.E. Six replicate were used for each treatment. The results of this study indicate that addition of fermacto prebiotic and Biotronic ® S.E increased the final body weight, significantly (p˂0.05). Furthermore, addition of additives decreased abdominal fat, triglycerides, serum cholesterol and increased carcass weight. Addition of fermacto prebiotic plus Biotronic ® S.E had the best performance, carcass characteristics and lowest serum cholesterol on broiler chickens at 42 day of age.