Proteinuria Enhances NHE3 Expression via a Mitochondrial Oxidative Stress/RAS Axis
Hong Kong Journal of Nephrology
Objective: To explore the effect of polarized macrophage 2c (M2c) on the activation of human renal proximal tubular epithelial cells. Methods: M2c were induced by treating with TPA (20 ng/ml) and IL-10 (20 ng/ml) for 0 h, 12 h, 24 h respectively, and then M2c were co-cultured with human kidney proximal tubular epithelial cells for 48 h. The mRNA and protein expression levels of fibrosis indexes. ɑ-SMA, E-cadherin and vimentin in proximal tubular epithelial cells were tested by RT-PCR,
... y RT-PCR, immunofluorescence and Western blotting. Migration assay was conducted to measure the migrated cells after co-cultured. Results: Compared with normal control group, the mRNA levels of M2c markers CXCL13, B7H4 were significantly increased (P < 0.01) after coculturing for 12 h and 24 h. Compared with normal control group, the mRNA and protein expressions of ɑ-SMA, vimentin were higher and E-cadherin were lower in the proximal tubular epithelial cells which were cocultured with M2c for 12 h and 24 h, especially in 24 h (P < 0.01). Migration assay indicated that the migration ability of proximal tubular epithelial cells was influenced by M2c, especially in 24 h (P < 0.01). Conclusion: Polarized M2c can induce the phenotype changes of human renal proximal tubular epithelial cells and increase the migration ability. Polarized M2c plays a role in the process of human renal proximal tubular epithelial cells activation.