Internet Archive Scholar

Influence of enzymes and extraction conditions on high yield of cottonseed milk

S. Thirukkumar, Department of Food Science and Nutrition, Community Science College and Research Institute, Tamil Nadu Agricultural University, Madurai- 625 104, India, G. Hemalatha, S. Vellaikumar, M. Murugan, S. Amutha, Department of Food Science and Nutrition, Community Science College and Research Institute, Tamil Nadu Agricultural University, Madurai- 625 104, India, Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai-625 104, India, Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore- 641 003, India, Department of Food Science and Nutrition, Community Science College and Research Institute, Tamil Nadu Agricultural University, Madurai- 625 104, India
2021 Journal of environmental biology  

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (1.2 MB)
https://web.archive.org/web/20210630040316/http://www.jeb.co.in/journal_issues/202107_jul21_spl/paper_15.pdf

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2021; you can also visit the original URL. The file type is application/pdf.

Aim: This research aimed to optimize suitable hydrolytic enzymes for maximizing cottonseed milk extracts for high cottonseed milk yield, protein content and low gossypol level. Methodology: Known amount of cottonseed was soaked for 90 min at 32°C and blended (cottonseed:water@1:6). Different aliquots of the blended cottonseed slurry were treated with 1% of enzymes viz., protease, cellulase and α-amylase enzyme at pH 7.0 followed by incubation at 40 and 52°C for 2.30 hr for the extraction of
more » ... onseed milk. The enzyme activity of extracted milk was subsequently inactivated by pasteurization (90°C, 5 min). Further analysis of physico-chemical characteristics was also carried. The control sample included milk extraction from non-enzyme treated cottonseed milk extract (30±2°C). Results: Among different treatments, cottonseed milk extraction using protease enzyme at 40°C incubation showed the highest milk yield (86.71%) with the lowest sedimentation (3.72%). Further incubation 40°C and 52°C showed the highest protein content of 2.10 and 2.27 g 100 ml-1 and gossypol reduction of 40.36 and 35.22%, respectively, in the cottonseed milk extract. Meanwhile, cellulase and α-amylase enzymes treated samples at both incubation temperatures showed poor physico-chemical characteristics as compared to control. Interpretation: Protease enzyme seems to be the most suitable for optimum or higher extraction of cottonseed milk.
doi:10.22438/jeb/42/4(si)/mrn-1584a fatcat:lhck4krskzhwpnxcq33w2ixugi
Open Access
Citation

S. Thirukkumar, Department of Food Science and Nutrition, Community Science College and Research Institute, Tamil Nadu Agricultural University, Madurai- 625 104, India, G. Hemalatha, S. Vellaikumar, M. Murugan, S. Amutha, Department of Food Science and Nutrition, Community Science College and Research Institute, Tamil Nadu Agricultural University, Madurai- 625 104, India, Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai-625 104, India, Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore- 641 003, India, Department of Food Science and Nutrition, Community Science College and Research Institute, Tamil Nadu Agricultural University, Madurai- 625 104, India. "Influence of enzymes and extraction conditions on high yield of cottonseed milk." Journal of environmental biology 42.4(SI) (2021) 1195-1200