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Peroxidase and Phosphatase Activity of Active-site Mutants of Vanadium Chloroperoxidase from the FungusCurvularia inaequalis
Journal of Biological Chemistry
Mutation studies were performed on active-site residues of vanadium chloroperoxidase from the fungus Curvularia inaequalis, an enzyme which exhibits both haloperoxidase and phosphatase activity and is related to glucose-6-phosphatase. The effects of mutation to alanine on haloperoxidase activity were studied for the proposed catalytic residue His-404 and for residue Asp-292, which is located close to the vanadate cofactor. The mutants were strongly impaired in their ability to oxidize chloridedoi:10.1074/jbc.275.16.11650 pmid:10766783 fatcat:khfn5qlpyzcmrdxflu4yb5a2vq