The main objective of this study is to evaluate the antimicrobial effect of ethanol solution extracts of G. dubia A. Terracc. on nineteen gram positive and negative bacteria (Bacillus subtilis, Enterococcus faecium, Enterococcus faecalis, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella infantis, Salmonella typhimurium, Salmonella kentucky, Salmonella enterica, Pseudomonas aeruginosa, Pseudomonas fluorescens) and one

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... ungi (Candida albicans). Different parts (flowers, leaves, stems, and roots) of Gagea dubia samples, were extracted with 80% ethanol solution. These extracts were tested in vitro for their antimicrobial activity against 15 microorganism by disk diffusion (DD) and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests. Ethanol extracts showed antimicrobial activity against some bacteria. According to the results of disk diffusion test G. dubia have effect on four bacteria (B. subtilis, S. aureus, L. innocua and E. durans) and (C. albicans) and didn't show effect on other microorganisms. The antibacterial effect observed in 10 µL extract of G.dubia against B. subtilis, S. aureus, L.innocua with 7,66; 13 and 9 mm zone diameter respectively and 50 µL extract of G. dubia against E.durans with 11 mm zone diameter. G. dubia extract show antimicrobial activity at three concentration (10;50 and 100 µL) against C. albicans between 9-15,66 mm zone diameter. MIC result for G. dubia determined as 5; 1,25; 10; 5 and 0,15 mg/100 µL against B. subtilis, S. aureus, L.innocua, E.durans and C. albicans respectively. According to MBC test all MIC value against maicroorganism determined as bacteriostatic.