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A High-Performance Liquid Chromatography method was developed for identification, quantification and control of possible impurities related to α-ketovaline calcium salt. A stainless-steel column (YMC Triart-C18: 250 × 4.6 mm, 5µm, made: Japan) and two different mobile phases [Mobile phase A containing 0.1% of orthophosphoric acid aqueous solution and B containing acetonitrile and methanol in a ratio 90:10 (v/v)] as an eluent at flow rate 1.0 mL/min were used to achieve the separation anddoi:10.31788/rjc.2021.1426272 fatcat:cerifzmjxzdrbbdhuwwkbgaawm