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Prm1 Functions as a Disulfide-linked Complex in Yeast Mating
2009
Journal of Biological Chemistry
Prm1 is a pheromone-induced membrane glycoprotein that promotes plasma membrane fusion in yeast mating pairs. HA-Prm1 migrates at twice its expected molecular weight on nonreducing SDS-PAGE gels and coprecipitates with Prm1-TAP, indicating that Prm1 is a disulfide-linked homodimer. The N terminus of a plasma membrane-localized GFP-Prm1 endocytic mutant projects into the cytoplasm, where it is protected from low pH quenching in live cells and from external protease in spheroplasts. In a revised
doi:10.1074/jbc.m109.068874
pmid:19933274
pmcid:PMC2807284
fatcat:abzveotprjbcdav22cgmiavsku