Dihydrofolate reductase (DHFR) reporter enzyme assay for Haloferax volcanii [post]

2019 unpublished
The dihydrofolate reductase (DHFR) is routinely used a reporter enzyme for H. volcanii. The DHFR catalyzes the reduction of dihydrofolate to tetrahydrofolate and the concomitant oxidation of NADPH to NADP + . This leads to a reduction of extinction at 340 nm, which is measured to quantify the DHFR activity. To avoid background, it is best to use an H. volcanii strain with a deletion of the chromosomal dhfr gene, which is available upon request
doi:10.21203/rs.2.11263/v1 fatcat:5jgdt5ss2vb6hpwniytt72udjy