Autophagy Assays (LC3B immunofluorescence, LC3B western blot, acridine orange assay)

Xin Zhang, Qingsong Liu
2013 Bio-protocol  
Autophagy is a dynamic cellular event that is involved in the degradation of long lived proteins and organelles in cells. Biochemical methods such as western blot to measure autophagic proteins have been increasingly used in autophagy studies because it is convenient and objective. Among them, the total amount of Microtubule-associated protein light chain 3 (LC3),
doi:10.21769/bioprotoc.1012 fatcat:ty7mqwsnofcm3axa3ifanrqkve