MEDICAL PROSPECTS AND MICRO PROPAGATION OF WITHANIA SOMNIFERA WITH TISSUE CULTURE TECHNIQUE IN LAB CONDITION

Ravinder Singh
2011 International Journal Of Pharmacy&Technology IJPT   unpublished
The vegetative micro propagation of flowering plants is meanly made by the cutting technique. However, this reproductive process not always presents satisfactory results, especially at times of the year when the plant lives an intensive vegetative development, this way affecting, its comercial production. Nowadays, the vegetative Micro propagation in ornamental plants has been represented as a propagation method that supplies a good shooting, rooting and other parts, without the necessity of a
more » ... hytoregulators application. The complete ringing technique was used in vegetative micro propagation of Withania somnifera. It was used completely randomized delineation and the data submieted to the variance analysis, by the Anova Test to a level of 5% of statistic probability. Each treatment consisted of three replications, analysed after 30 days of the installation of the experiment. It was conclued that the vegetative micro propagation practice promoted enough rooting, shooting & other plant parts in all substratums, and the organic soil the one that supplied the most development of the roots. The advantage of vegetative micro propagation are, to obtain a high degree of crop uniformity to overcome complex dormacy problem, seedling grwth, low seed viability and difficulty in procedures dependent upon micro propagation. The main advantage of tissue culture technology lies in the production of high qualitity and uniform planting material on a year-round basic under disase-free condition anywhere irrespective of the season and weather.
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