Investigation of Resistance and Clonal Relatedness Among The Nosocomial Acinetobacter Isolates

Nilay Ildız
2019 MIDDLE BLACK SEA JOURNAL OF HEALTH SCIENCE  
Objective: This study focused on Acinetobacter spp. that were isolated from inpatients to determine the resistance rates to antibiotics and to monitor the resistance increase over the years. Method: To determine whether there was a clonal relationship between the strains using pulsed field gel electrophoresis (PFGE), to evaluate the epidemiological and clinical data of patients, and to determine the factors that may be effective in the formation and spread of infection, antibiotic
more » ... es of Acinetobacter strains were evaluated by the Kirby Bauer disk diffusion method. Molecular typing was studied with PFGE. Results: Netilmicin had the least resistance (7%) among the strains, while the highest resistance was found against piperacillin (92%), ceftriaxone (81%), and doxycycline (69%). When resistance profiles of strains were compared according to year (2005)(2006)(2007), no significant changes were found for resistance rates to piperacillin, ceftriaxone, gentamicin, doxycycline, and trimethoprim / sulfamethoxazole. Of all the 131 tested Acinetobacter spp., 82 (62.6%) of the strains were clustered with the PFGE method and 72.3% of the strains were clonally related. The increase in MDR, XDR and PDR rates among the isolated Acinetobacter spp. is remarkable. It was determined that the degree of transmission of the strains was quite high among patients. The clone in the hospital was able to remain in the environment for long periods and the general patient mortality rates were higher than other studies. Conclusions: According to results and with the support of molecular typing studies, the need for more effective prevention and control measures in our hospital was demonstrated.
doi:10.19127/mbsjohs.581706 fatcat:effluaha4japdenti2vdz6223a