Fluconazole and amphotericin B antifungal susceptibility testing by National Committee for Clinical Laboratory Standards broth macrodilution method compared with E-test and semiautomated broth microdilution test

J L Rodríguez-Tudela, J V Martinez-Suarez
1997 Journal of Clinical Microbiology  
Author's Reply The remarks of my colleagues and I regarding the effect of glucose addition on endpoint reading were based on the following experiment. MIC endpoints for fluconazole in RPMI and RPMI plus glucose were determined for 16 C. glabrata, 10 C. albicans, 2 C. krusei, and 2 C. parapsilosis isolates. RPMIglucose was prepared as described elsewhere (1). Addition of glucose to drug-free medium improved growth of C. glabrata and C. albicans isolates but not that of the C. krusei or C.
more » ... losis isolates (mean absorbance readings after 22 h in RPMI, 0.85 versus 1.55 in RPMI-glucose [C. glabrata] and 0.7 versus 1.2 [C. albicans]). Addition of glucose had the same stimulatory effect on growth in medium with fluconazole at subinhibitory concentrations. MIC endpoints obtained in RPMI were not different from those obtained in RPMI-glucose. In addition, based on the relative distance between the endpoints, addition of glucose did not lead to a better separation between 80%-or-greater growth inhibition endpoints and 336 on May 7, 2020 by guest
doi:10.1128/jcm.35.1.336-337.1997 fatcat:ocq6c5q2fvetfks3tfjmkx6ple