A method for high-throughput gene expression signature analysis

David Peck, Emily D Crawford, Kenneth N Ross, Kimberly Stegmaier, Todd R Golub, Justin Lamb
2006 Genome Biology  
Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a
more » ... ion based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.
doi:10.1186/gb-2006-7-7-r61 pmid:16859521 pmcid:PMC1779561 fatcat:pe3riqtkdvg65fyqa4urf6dssu