Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories

Alexandria E. Kesterson, John E. Craig, Lara J. Chuvala, Henry S. Heine
2020 Emerging Infectious Diseases  
The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that
more » ... ety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories.
doi:10.3201/eid2611.191630 pmid:33079040 fatcat:r46c5q633nhnlabinfm5vt2xqm