The bacteriology of peridental tissues radiographically suggesting infection

C. Coleman Berwick
1921 Journal of Infectious Diseases  
The medical and dental literature for a number of years has been filled with reports and bacteriologic data concerning chronic alveolar infections. A critical review of the wealth of material reveals one oustanding feature, namely, that no single report is based on a uniform and reliable bacteriologic technic. This applies especially to the method of obtaining cultures. Moreover, the reports indicate a 1<-Ick of standardized operative technic and some form of statement which would prove that
more » ... efully conceived series of controls have been clrr;ed out. It is not unlikely that the cultures obtained and described (l.t some length in certain publications were merely contaminations from the oral flora. For this reason I have undertaken to investigate the bacteriology of chronic alveolar infections as evidenced by shadow changes in the radiogram; to determine the relationship of the cultures obtained to the bacteria of the oral cavity; to ascertain, if possible, the relationship of chronic alveolar infections to systemic disease. Method of Stud)I.-In a previous article 1 1 reported a method of sterilizing the oral mucosa which proved satisfactory not only from the laboratory point of view, but also from the clinical aspect. The cultures were made from tissues dissected from jaws showing definite radiographic changes. The material was obtained as follows: The teeth were first brushed with an alkaline tooth powder and then the mouth was rinsed with an alkaline lotion. The alkaline reaction of the tooth preparation intensifies the bacteriostatic action of the dye. The gums and mucous membrane of the buccal surfaces were painted with a solution containing 1% each of brilliant green and crystal violet, dissolved in 50% alcohol. Churchman has shown that it is possible to inhibit the growth of gram-positive cocci in dilution of gentian violet up to 1 : 5,000,000 in alkaline solution. Hence, in every instance care was taken to avoid a transfer of dye into the culture medium with
doi:10.1093/infdis/29.5.537 fatcat:xrjz222o3rcyhcavflf2f6gjdu