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The electrical threshold of A-V nodal cells of isolated rabbit heart preparations was estimated by techniques which permitted stimulation and recording through the same micropipette. Cells within the node were significantly less excitable than adjacent atrial and ventricular tissues; thresholds exceeded 5 X 10~~° amp in some instances. Recovery of excitability was delayed well beyond repolarization; in cells of the midnodal region, "diastolic" threshold was not attained until 0.2 to 0.5 secondsdoi:10.1161/01.res.23.1.69 pmid:5661940 fatcat:psq22jqreba2rbqhwb2tgpzdvu