Human neonatal lymphocytes immortalized after microinjection of Epstein-Barr virus DNA

C Klein, N Raab-Traub
1987 Journal of Virology  
Epstein-Barr virus (EBV) is a highly efficient acute transforming agent in human cells, provided that the intact virus is used. To investigate the ability of viral DNA alone to transform cells, we introduced the EBV genome into human lymphocytes. After microinjection of EBV DNA into neonatal B lymphocytes, we established a cell line that in early passages contained multiple viral fragments. This cell line retained sequences from the short, unique (U,) region of the EBV genome and sequences from
more » ... EcoRI-E. The viral sequences were not expressed; however, the cells expressed a 2.3-kilobase polyadenylated message homologous to the c-fgr oncogene, a cellular locus believed to be activated by EBV infection [
doi:10.1128/jvi.61.5.1552-1558.1987 fatcat:crprv72u2fb3rdxyotjqfggwae