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Nitrile hydratase, which occurs abundantly in cells of Rhodococcus rhodochrous Jl isolated from soil samples, catalyzes the hydration of 3-cyanopyridine to nicotinamide. By using resting cells, the reaction conditions for nicotinamide production were optimized. Under the optimum conditions, 100% of the added 12 M 3-cyanopyridine was converted to nicotinamide without the formation of nicotinic acid, and the highest yield achieved was 1,465 g of nicotinamide per liter of reaction mixturedoi:10.1128/aem.54.7.1766-1769.1988 fatcat:4xextufkajayjowq533kyf4vau