Direct electrochemical detection of trichothecenes in wheat samples using a 96-well electrochemical plate coupled with microwave hydrolysis

F. Ricci, F. Pino, M. Abagnale, M. Messia, E. Marconi, G. Volpe, D. Moscone, G. Palleschi
2009 World Mycotoxin Journal  
The use of a 96-well electrochemical plate for the fast and sensitive detection of deoxynivalenol and nivalenol in wheat samples is shown. Deoxynivalenol and nivalenol are hydrolysed using a microwave hydrolysis procedure (2 min) which leads to the production of electroactive compounds that can be sensitively detected by the use of cheap screen-printed electrodes. A procedure of extraction with aqueous acetonitrile and a clean-up step was demonstrated to be suitable for the application with
more » ... pplication with wheat samples providing suitable detection limit (LOD=1.1 µg/g) and working range (2-20 µg/g) for the determination of deoxynivalenol in cereals for feed consumption.
doi:10.3920/wmj2008.1133 fatcat:n34eucxvgnephlswx5vukrf744