Peer Review #1 of "High-throughput sequence analysis reveals variation in the relative abundance of components of the bacterial and fungal microbiota in the rhizosphere of Ginkgo biloba (v0.1)" [peer_review]

2019 unpublished
Background. The narrow region of soil, in contact with and directly influenced by plant roots, is called the rhizosphere. Microbes living in the rhizosphere are considered to be important factors for the normal growth and development of plants. In this research, the structural and functional diversities of microbiota between the Ginkgo biloba root rhizosphere and the corresponding bulk soil were investigated. Methods. Three independent replicate sites were selected, and triplicate soil samples
more » ... ere collected from the rhizosphere and the bulk soil at each sampling site. The communities of bacteria and fungi were investigated using high-throughput sequencing of the 16S rRNA gene and the internal transcribed spacer (ITS) of the rRNA gene, respectively. Results. A number of bacterial genera showed significantly different abundance in the rhizosphere compared to the bulk soil, including Bradyrhizobium, Rhizobium, Sphingomonas, Streptomyces and Nitrospira. Functional enrichment analysis of bacterial microbiota revealed consistently increased abundance of ATP-binding cassette (ABC) transporters and decreased abundance of two-component systems in the rhizosphere community, compared to the bulk soil community. In contrast, the situation was more complex and inconsistent for fungi, indicating the independency of the rhizosphere fungal community on the local microenvironment. Abstract 18 Background. The narrow region of soil, in contact with and directly influenced by plant roots, is 19 called the rhizosphere. Microbes living in the rhizosphere are considered to be important factors 20 for the normal growth and development of plants. In this research, the structural and functional 21 diversities of microbiota between the Ginkgo biloba root rhizosphere and the corresponding bulk 22 soil were investigated. 23 Methods. Three independent replicate sites were selected, and triplicate soil samples were 24 collected from the rhizosphere and the bulk soil at each sampling site. The communities of 25 bacteria and fungi were investigated using high-throughput sequencing of the 16S rRNA gene 26 and the internal transcribed spacer (ITS) of the rRNA gene, respectively. 27 Results. A number of bacterial genera showed significantly different abundance in the 28 rhizosphere compared to the bulk soil, including Bradyrhizobium, Rhizobium, Sphingomonas, 29 Streptomyces and Nitrospira. Functional enrichment analysis of bacterial microbiota revealed 30 consistently increased abundance of ATP-binding cassette (ABC) transporters and decreased 31 abundance of two-component systems in the rhizosphere community, compared to the bulk soil 32 community. In contrast, the situation was more complex and inconsistent for fungi, indicating the 33 independency of the rhizosphere fungal community on the local microenvironment.
doi:10.7287/peerj.8051v0.1/reviews/1 fatcat:yohbehlmz5cnxgenky3ygly6e4