Using arsenazo-III as chromogenic reagent, a spectrophotometric method for the determination of nickel was developed. The maximum absorption wavelength of nickel complex is 610 nm. Beer's law is conformed over the range of 0.2 ~ 4.0 µ g / mL for nickel. The apparent molar absorption coefficient of method is ε 540 nm = 1.76 × 10 4 L·mol-1 ·cm-1 and detection limit is 0.093 µ g / mL. The present method has been already applied to the determination of nickel in tea and method recovery is between 99.7% ~ 100.1%.