Figures S1 and S2 from tagPAINT: covalent labelling genetically encoded protein tags for DNA-PAINT imaging

Daniel J. Nieves, Geva Hilzenrat, Jason Tran, Zhengmin Yang, Hugh H. MacRae, Matthew A. B. Baker, J. Justin Gooding, Katharina Gaus
2019 Figshare  
Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.
doi:10.6084/m9.figshare.11300690 fatcat:6hgxvpwc45cjldtync5vhbxndu