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The enzyme β-galactosidase (β-D-galactoside galactohydrolase, EC 220.127.116.11) commonly known as lactase, has important applications in the dairy industry. From culture of strain Lactobacillus acidophilus having high and stable β-galactosidase activity, the study of crude enzyme isolation were carried out by ultrasonical extraction and precipitation by neutral salts and organic solvents. Best precipitant was isopropanol with enzyme recovery 89,93%, and enzyme purity increased 4,5 folds. Furtherdoi:10.32508/stdj.v15i3.1818 fatcat:utaazywba5c7necyvmq2gpto4i