Isolation and Characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic Cod (Gadus morhua)

Robert Tylingo, Szymon Mania
2016 Journal of Biotechnology & Biomaterials  
Citation: Tylingo R, Mania S, Panek A, Piątek R, Pawłowicz R (2016) Isolation and Characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic Cod (Gadus morhua). J Biotechnol Biomater 6: 234. Abstract Acid-soluble collagen (ASC) from the fish skin of African catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic cod (Gadus morhua) was extracted and characterized. The ASC extraction yield was 75%, 73% and 68%,
more » ... and 68%, respectively. The denaturation and melting temperatures of African catfish ASC (29.3°C and 100.0°C) were significantly higher than ASC of Salmon and Baltic cod (20.6°C and 90.5°C; 15.2°C and 86.7°C, respectively), assessed by differential scanning calorimetry. The SDS-PAGE profile showed that each of tested ASC was the type I collagen and consisted of two different α chains, α1 and α2, as well as a β component. The FTIR spectra of all collagens indicate that the overall their chemical compositions are quite similar. The fish skin collagen is easy to prepare and represents a possible resource for use on industrial scale. Citation: Tylingo R, Mania S, Panek A, Piątek R, Pawłowicz R (2016) Isolation and Characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic Cod (Gadus morhua). Volume 6 • Issue 2 • 1000234 J Biotechnol Biomater ISSN: 2155-952X JBTBM, an open access journal Citation: Tylingo R, Mania S, Panek A, Piątek R, Pawłowicz R (2016) Isolation and Characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic Cod (Gadus morhua). Volume 6 • Issue 2 • 1000234 J Biotechnol Biomater ISSN: 2155-952X JBTBM, an open access journal Citation: Tylingo R, Mania S, Panek A, Piątek R, Pawłowicz R (2016) Isolation and Characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic Cod (Gadus morhua). Volume 6 • Issue 2 • 1000234 J Biotechnol Biomater ISSN: 2155-952X JBTBM, an open access journal Citation: Tylingo R, Mania S, Panek A, Piątek R, Pawłowicz R (2016) Isolation and Characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic Cod (Gadus morhua).
doi:10.4172/2155-952x.1000234 fatcat:ol3xhkgcgnbt5og6gbaztvl3vi