Stable Immobilization of Enzyme on Pendant Glycidyl Group-Modified Mesoporous Carbon by Graft Polymerization of Poly(Glycidyl Methacrylate)

Isao Shitanda, Takanao Kato, Ryo Suzuki, Tatsuo Aikawa, Yoshinao Hoshi, Masayuki Itagaki, Seiya Tsujimura
2019 Bulletin of the Chemical Society of Japan  
Poly(glycidyl methacrylate) (poly(GMA)) bearing pendant glycidyl groups, grafted on the surface of MgO-templated carbon (MgOC), is useful for forming strong multipoint covalent bonds with amino functional groups on the surface of flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) molecules. The immobilized FAD-GDH can generate glucose-oxidation catalytic current using 1,2-naphthoquinone (12NQ) as the redox mediator, which is also captured on the electrode surface. The
more » ... c current is more stable than that obtained using the FAD-GDH-MgOC electrode without poly(GMA) because the leaching of FAD-GDH and 12NQ is suppressed by the enzyme capping effect.
doi:10.1246/bcsj.20190212 fatcat:hyazsefeh5gfpec4c43gs5h36a