First report of a ' Candidatus Phytoplasma asteris' strain affecting lingonberry ( Vaccinium vitis‐idaea ) and causing lingonberry stunt phytoplasma disease

A.‐S. Brochu, A. Methot, A.‐M. Breton, C. Lacroix, J.‐P. Légaré, E. Pérez‐López
2022 New Disease Reports  
Vaccinium vitis-idaea is a red berry-producing woody shrub, native to North America, Europe, and Scandinavia, known commonly as lingonberry (Hendrickson, 1997) . During the spring/summer seasons of 2019 and 2020, producers from Quebec, Canada, have noticed symptoms of yellowing leaves and stunted growth, resembling symptoms caused by phytoplasma. To confirm whether phytoplasmas were indeed associated with these reported symptoms, fifteen lingonberry plants from different producers from the
more » ... n of Chaudière-Appalaches were analysed (Table 1 ). Of these, nine had symptoms of stunting, little leaves and yellowing, while the rest showed no visible symptoms when collected (Figure 1 ). For each plant, total DNA was extracted from leaves and stem tissue using a CTAB-based method. The DNA was used as a template for a cpn60UT PCR with primers H279p/H280p (Dumonceaux et al., 2014) , and the positive samples were subjected to direct PCR with phytoplasma 16S primers R16F2n/R16R2 as previously described (Perez-Lopez et al., 2019) . Amplicons of the expected size (∼600 bp for cpn60UT) were obtained for 9/15 samples, while those for R16F2n/R2 sequences were obtained for 8/9 samples positives using the cpn60UT assay (Table 1 ). The cpn60UT and R16F2n/R2 amplicons obtained This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
doi:10.1002/ndr2.12058 fatcat:i5arfgscm5c3nc77xb2lbaysby