Gd-BOPTA Transport Into Rat Hepatocytes: Pharmacokinetic Analysis of Dynamic Magnetic Resonance Images Using a Hollow-Fiber Bioreactor

Corinne Planchamp, Marianne Gex-Fabry, Christophe Dornier, Rafael Quadri, Marianne Reist, Marko K. Ivancevic, Jean-Paul Vall??e, Sibylle Pochon, Fran??ois Terrier, Luc Balant, Bruno Stieger, Peter J. Meier (+1 others)
2004 Investigative Radiology  
Rationale and Objectives: To investigate the transport of the hepatobiliary magnetic resonance (MR) imaging contrast agent Gd-BOPTA into rat hepatocytes. Materials and Methods: In a MR-compatible hollow-fiber bioreactor containing hepatocytes, MR signal intensity was measured over time during the perfusion of Gd-BOPTA. For comparison, the perfusion of an extracellular contrast agent (Gd-DTPA) was also studied. A compartmental pharmacokinetic model was developed to describe dynamic signal
more » ... ty-time curves. Results: The dynamic signal intensity-time curves of the hepatocyte hollow-fiber bioreactor during Gd-BOPTA perfusion were adequately fitted by 2 compartmental models. Modeling permitted to discriminate between the behaviors of the extracellular contrast agent (Gd-DTPA) and the hepatobiliary contrast agent (Gd-BOPTA). It allowed the successfully quantification of the parameters involved in such differences. Gd-BOPTA uptake was saturable at high substrate concentrations. Conclusions: The transport of Gd-BOPTA into rat hepatocytes was successfully described by compartmental analysis of the signal intensity recorded over time and supported the hypothesis of a transporter-mediated uptake.
doi:10.1097/01.rli.0000129156.16054.30 pmid:15257212 fatcat:2pbm5sitbbakpbbpw36nxrenau