Efficient electrospray ionization from polymer microchannels using integrated hydrophobic membranes

Ying-Xin Wang, Jon W. Cooper, Cheng S. Lee, Don L. DeVoe
2004 Lab on a Chip  
A simple process for realizing stable and reliable electrospray ionization (ESI) tips in polymer microfluidic systems is described. The process is based on the addition of a thin hydrophobic membrane at the microchannel exit to constrain lateral dispersion of the Taylor cone formed during ESI. Using this approach, ESI chips are shown to exhibit well-defined Taylor cones at flow rates as low as 80 nL min 21 through optical imaging. Furthermore, stable electrospray current has been measured for
more » ... been measured for flow rates as low as 10 nL min 21 over several hours of continuous operation. Characterization of the electrospray process by optical and electrical monitoring of fabricated ESI chips is reported, together with mass spectrometry validation using myoglobin as a model protein. The novel process offers the potential for low-cost, direct interfacing of disposable polymer microfluidic separation platforms to mass spectrometry.
doi:10.1039/b402825b pmid:15269805 fatcat:4fgin4jm6va3xpku7esf54drri