Cryptosporidium from water sample in Bangladesh

Sharifuzzaman, Anisuzzaman, Md Shafiullah Parvez, Antonio Barragan
2015 International Journal of Natural and Social Sciences   unpublished
Water-borne cryptosporidiosis, a neglected parasitic disease, presents a serious threat to human health due to the ubiquitous distribution of Cryptosporidium species affecting humans and animals, and due to the resistance of the oocysts to harsh environmental conditions, various disinfectants, and some common therapeutic agents. Water is a great reservoir for contamination and a potential medium of transmission of the pathogen. The present study was aimed to isolate and identify Cryptosporidium
more » ... ify Cryptosporidium oocysts in water samples in Bangladesh. Water samples from small ponds, waste water bodies and drains were collected from different areas of the Mymensingh and Kushtia districts of Bangladesh. To detect Cryptosporidium, the samples were assessed by microscopy, using the conventional Ziehl-Neelsen staining method, and by PCR. By microscopy, Cryptosporidium oocysts were detected in 5 (25%) of 20 samples examined collected from the Mymensingh district, which were further confirmed by PCR. Importantly, Cryptosporidium was detected by PCR in drain water but was not detected by conventional microscopy. In the Kushtia district, 2 (20%) of the 10 samples examined by microscopy were positive. Cryptosporidium was not detected in waste water by conventional microscopy and PCR. However, the results confirm the presence of Cryptosporidium in different types of sources of water in both Mymensingh and Kushtia districts, demonstrating the existence of Cryptosporidium in the study areas. Further studies are need to explore the present status of prevalence of Cryptosporidium in humans and animals in order to better understand the transmission dynamics of the parasite and thereafter take necessary measures to control and/or prevent the disease.