monoclonal antibody for detection of Leptosphaeria korrae, the causal agent of necrotic ringspot disease of turfgrass. Phytopathology 80:1208-1211. Monoclonal antibody (MAb) LKc50 was developed against Lepto-mudagrass. MAb LKc50 reacted negatively to 38 of 42 isolates of resphaeria korrae strain ATCC 56289. The antibody was capable of detecting lated and nonrelated fungi and negatively to apparently healthy grass. L. korrae from cultures and in naturally infected Kentucky bluegrass The limit of

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... detection was less than 2 Mg/ ml of lyophilized mycelial homosamples from three states. In cross-reactivity tests using indirect ELISA, genate. MAb LKc50 provides a means for rapid detection of L. korrae, MAb LKc50 reacted positively to all 24 isolates of L. korrae screened, an ectotrophic root-invading fungus that is difficult to identify using conincluding strains from six states, from both Kentucky bluegrass and ber-ventional methods.