DISCOVERY OF A NEW PRINCIPLE (Sub-rubrine) IN HUMAN BLOOD, IN HEALTH AND DISEASE, AND ALSO IN THE BLOOD OF SEVERAL OF THE LOWER MAMMALIA

W OSHAUGHNESSY
1835 The Lancet  
677 buttocks elevated. The tumour is coverad with a piece of fine linen, covered with ointment or cold cream. General and uniform pressure is made upon the protrusion, so as to diminish its bulk. The forefinger of the right or left hand is then pushed into the orifice, carrying the cloth with it. A silver canula is recommended by old BENJAMIN BELL, a card made into a cone by Sir CHARLES. In withdrawing the finger, care is taken, by the position t of the other fingers round the margin, to
more » ... e margin, to prevent the escape of the folds again. The le parts are easily retained by compress and bandage. Some who labour under this disease, find advantage from wearing a sort of truss, with a stuffed or ivory pad, and a kind of pessary, or short bougie, is also used. These means are merely palliative, if even that can be said of them, or of the bougies and plugs which some of the rectum doctors would have us poke in on all occasions for the prolapsus, and all other sort of real or imagined vice in the parts. As a radical cure, the operation directed is quite effectual when properly gone about. It is certainly much less painful than the barbarous practice of applying caus-A FEW days before my departure from Calcutta in April last, while engaged in the analysis of some specimens of blood drawn from patients labouring under disease of the spleen, my attention was forcibly attracted by some very remarkable phenomena, which were wholly inexplicable according to the previous state of our knowledge of the composition of the blood. These appearances surprised me the more, as in the course of my inquiries regarding the chemical pathology of the cholera, I was necessarily obliged to subject nearly two hundred samples of blood to a rigorous analysis, conducted chiefly according to the processes recommended by Lecanu and Denis, whose works are the latest and best authorities on this in-I teresting subject. The appearances I allude to first pre-sented themselves during an experiment made to ascertain the amount of colouring matter in 1000 grains of spleen blood. Being pressed for time, I adopted a mode of analysis calculated to afford more expeditious results than that I was previously in the habit of employing. It consisted in decanting the serum, and depriving the coagulum of its fibrine by kneading it in a muslin bag. Alcohol was then added with a view to coagulate and throw down the colouring matter and adhering albumen. The precise amount of this albumen being readily known by data. afforded bv the analysis of the serum, the amount of pure colouring matter can thus be precisely computed. WhEn the alcohol was added, and the coagulation effected, I threw the mixture on a very fine muslin filter, a very turbid fiuicl immediately passed through. Supposing that this was merely imperfectly coagulated colouring matter, I boiled the turbid fluid in order to accelerate the separation I expected ; to my surprise, however, instead of this effect, the very contrary was produced. The heated fluid, instead of coagulating, became more transparent, and all the turbid flocculi were dissolved when the boning Domi. was attamea. Allowed to cool, the solution again became cloudy, and when at 80 Fahrenheit, a copious deposit of a faint flesh colour was obtained. By alternate heating and cooling, whether gradual or sudden, the same effects were indefinitely produced. A portion was filtered and dried, in which state it was soluble in dilute alcohol, infusible when heated on platinum foil, and insoluble in sulphuric ether. To those practically conversant with this department of animal chemistry, I need scarcely observe, that the preceding facts afforded by themselves the strongest evidence of the existence of a principle previously unknown in the blood then under examination. But as several gentlemen, who may hear or read this paper, are much more profitably employed than in the pursuits of practical analysis, I will venture to explain these proofs of the specialty of the principle in question. The animal principles previously known
doi:10.1016/s0140-6736(02)96760-7 fatcat:zr55kyxvjngjdfyrdplt6tt76m