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The intracellular environment affects protein–protein interactions
Proceedings of the National Academy of Sciences of the United States of America
Protein–protein interactions are essential for life but rarely thermodynamically quantified in living cells. In vitro efforts show that protein complex stability is modulated by high concentrations of cosolutes, including synthetic polymers, proteins, and cell lysates via a combination of hard-core repulsions and chemical interactions. We quantified the stability of a model protein complex, the A34F GB1 homodimer, in buffer, Escherichia coli cells and Xenopus laevis oocytes. The complex is moredoi:10.1073/pnas.2019918118 pmid:33836588 fatcat:g5aepg66tjawbcflom3qq6njcu