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Identification of new 16SrIX subgroups, -F and -G, among 'Candidatus Phytoplasma phoenicium' strains infecting almond, peach and nectarine in Lebanon

Marina MOLINO LOVA, Fabio QUAGLINO, Yusuf ABOU-JAWDAH, Elia CHOUEIRI, Hana SOBH, Paola CASATI, Rosemarie TEDESCHI, Alberto ALMA, Piero BIANCO
2011 Phytopathologia Mediterranea  

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Almond witches'-broom is a lethal almond disease associated with 'Candidatus Phytoplasma phoenicium', subgroups 16SrIX-B and -D. In Lebanon, where the disease was first reported, analogous diseases affecting peach and nectarine were recently associated with presence of 'Ca. P. phoenicium'. In the present study, genetic diversity among 24 'Ca. P. phoenicium' strains infecting almond, peach and nectarine plants in diverse geographic regions of Lebanon was investigated by virtual restriction
more » ... nt length polymorphism (RFLP) analysis of 16S rDNA nucleotide sequences. Calculation of virtual restriction similarity coefficients revealed the presence of two new subgroups, -F and -G, in group 16SrIX that were confirmed by real RFLP analyses. Obtained results open new opportunities for in-depth studies of the distribution of 'Ca. P. phoenicium' strains in plant hosts and insect vector populations from different geographic areas of Lebanon.
doi:10.14601/phytopathol_mediterr-9253 doaj:f16b600d47c4466da8f9781456b240fc fatcat:3jvdsgkugramvk4q2km2yofw7a
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Marina MOLINO LOVA, Fabio QUAGLINO, Yusuf ABOU-JAWDAH, Elia CHOUEIRI, Hana SOBH, Paola CASATI, Rosemarie TEDESCHI, Alberto ALMA, Piero BIANCO. "Identification of new 16SrIX subgroups, -F and -G, among 'Candidatus Phytoplasma phoenicium' strains infecting almond, peach and nectarine in Lebanon." Phytopathologia Mediterranea (2011)