Mechanisms involved in uptake of Bordetella bronchiseptica by mouse dendritic cells

C A Guzman, M Rohde, K N Timmis
1994 Infection and Immunity  
The invasion and intracellular survival ofBordeteUla bronchiseptica in mouse dendritic cells were investigated. The results obtained suggest that B. bronchiseptica binds specifically to glycosylated receptors present on the plasma membrane of dendritic cells, thereby inducing a signal that triggers an actin polymerization-dependent phagocytic process, probably via a protein kinase-dependent transducing phosphorylation signal. The energy required for the uptake process by host cells is provided
more » ... cells is provided mainly by the glycolytic pathway. An intact microtubule system and de novo protein synthesis in eukaryotic and prokaryotic cells are essential for efficient uptake and intracellular survival. The interaction of B. bronchiseptica with dendritic cells may be pertinent to natural infections that follow a chronic clinical course and predispose to secondary infections, and to the T-cell response involved in protective immunity following infections caused by Bordetella spp. Bordetella bronchiseptica is associated with different pathological syndromes characterized mainly by a mild and chronic clinical course in several animal species (21, 34, 59) . Traditionally, the toxins and adhesins produced by these microorganisms were considered the main factors involved in pathogenesis of Bordetella spp. (3, 21, 23, 27, 34, 47, 59) , although an increasing body of evidence supports a general invasive capability for B. bronchiseptica (50, 51) and other Bordetella spp. (5, 8, 11, 13, 17, 33, 46, 49) . Particularly significant in the pathogenesis of Bordetella pertussis appear to be invasion and intracellular survival in macrophages (8, 17, 49). We have recently reported that B. bronchiseptica is efficiently internalized by mouse dendritic cells (DC) and survives intracellularly for at least 72 h (25). The interaction with DC could be relevant in the infective processes caused by B. bronchiseptica since DC are present in the airways (38, 53), the portal of entry for B. bronchiseptica, and are the most efficient antigenpresenting cells (57, 58) . This interaction might explain, at least in part, the chronic clinical course of the disease, the secondary infections that commonly occur, and the type of immune response elicited during natural infection. In the present study, a fully functional murine spleen DC line was used to characterize the mechanisms of bacterial uptake and persistent intracellular survival. MATERIALS AND METHODS Bacterial strains and media. The wild-type B. bronchiseptica 5376 was used throughout this work (60) and cultivated at 37°C on Bordet-Gengou agar base (Difco Laboratories, Detroit, Mich.) supplemented with 1% glycerol and 15% (vol/vol) defibrinated horse blood, in SS broth (54), and in brain heart infusion broth or agar (Difco Laboratories). Liquid cultures were aerated by shaking at 300 rpm in a New Brunswick Environmental Incubator Shaker.
doi:10.1128/iai.62.12.5538-5544.1994 fatcat:wppsd3pvnnhlte63nltfcuhp7u