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This study aims to identify the vibrio in mangrove forest areas near to the location of vannamei ponds, especially in sediment substrates. The research is at the Mangunharjo sub-district in the Mayangan District, Probolinggo City, East Java Province. The time of study is august and September 2019. Isolation of bacteria being done with total plate count procedure on Thio Sulfate Citrate Bile Sucrose (TCBS) Agar, with pour plate method. Morphology characterization and the biochemical test wasdoi:10.5281/zenodo.3526857 fatcat:mtzo43qzvvbahibrfvlpsrkmry