Molecular Genetic Markers and Biochemical Detection between Two Capparis sp

A.H. Hassan, Esraa A. El Sherbeny
2016 IOSR Journal of Agriculture and Veterinary Science  
This investigation was carried out to assess the relationships between two Capparis sp. (Capparis cartilaginea and Capparis spinosa). Molecular markers Random amplified polymorphic DNA (RAPD) and the inter-simple sequences repeat (ISSR) markers technique were used to determine the genetic relationships between two Capparis sp. The results suggest that a RAPD marker is the best choice for the evaluation of diversity and the genetic relationships between two Capparis sp. with high accuracy which
more » ... igh accuracy which revealed 183 band DNA bands detected across sixteen primers with high polymorphism than ISSR which revealed 76 DNA bands. The genetic relationship among eight genotypes of two Capparis sp. based on biochemical and molecular data developed using dendrogram constructed by UPGMA cluster Conservationist may use the information of the present study to make effective decisions regarding the global protection and management of two Capparis sp. in Egypt.
doi:10.9790/2380-0908014858 fatcat:y4s5etm7yfh6fb6unmmk3cjwza