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An analytical method was developed and validated for separation, detection and quantification of patulin in apple cider by high-performance liquid chromatography (HPLC-DAD). Extraction and clean-up of patulin from apple cider are achieved on AFFINIMIP� SPE PATULIN cartridge (3mL/100mg). Patulin was separated on a C18 Hypersil GOLD column (150 mm � 4 mm, 5 mm) coupled to a Hypersil Gold guard column (10 � 4.0 mm, 5 mm) using mobile phase consisting of water:acetonitrile (95:5, v/v) and detecteddoi:10.37358/rc.19.11.7673 fatcat:tbgpyklfezb6bbdmmtf5de52fm