Internal Validation of Rapid and Performance Method for Patulin Determination in Apple Cider by High-Performance Liquid Chromatography

Monica Catana, Luminita Catana, Enuta Iorga, Adrian Constantin Asanica, Anda Gratiela Lazar, Monica Alexandra Lazar, Nastasia Belc, Gina Pirvu
2019 Revista de chimie (Bucuresti)  
An analytical method was developed and validated for separation, detection and quantification of patulin in apple cider by high-performance liquid chromatography (HPLC-DAD). Extraction and clean-up of patulin from apple cider are achieved on AFFINIMIP� SPE PATULIN cartridge (3mL/100mg). Patulin was separated on a C18 Hypersil GOLD column (150 mm � 4 mm, 5 mm) coupled to a Hypersil Gold guard column (10 � 4.0 mm, 5 mm) using mobile phase consisting of water:acetonitrile (95:5, v/v) and detected
more » ... v/v) and detected at 276 nm. The method has a good sensitivity (LOD = 2.63 mg/mL and LOQ = 8.45mg/mL) and a good precision (RDS (r) = 0.32...0.91% for injection repeatability; RSD (r) = 1.05�.2.98% for analysis repeatability intra-day; RSD (R) = 1.35�.3.24% for intermediate precision; RSD (R) = 1.64�.3.86% for intra-laboratory reproducibility). The method was applied by analyzing samples of apple cider purchased from commerce. In case of 80% of the analyzed samples, patulin was not detected, and for the rest of them concentration of patulin was [ LOQ.
doi:10.37358/rc.19.11.7673 fatcat:tbgpyklfezb6bbdmmtf5de52fm