Morphofunctional and Metabolic Alterations after Orchiectomy

Alberti Luiz Ronaldo
2016 Open Journal of Biological Sciences  
Objective: To verify alterations in lipid profile, blood glucose and pressure, and morphological aspects of the adrenal gland in animals with hypogonadism secondary to total bilateral epididymectomy and orchiectomy. Methods: Young and adult male Wistar rats were distributed into the following groups: Group 1 -control, animals submitted only to scrotal incision and suture. Group 2 -animals submitted to total bilateral epididymectomy and orchiectomy. After these operations, studies were carried
more » ... t to analyze possible repercussions of hypogonadism. In Study A, serum levels of free testosterone were measured, along with the body weight and cortical thickness of the adrenal gland of 30 adult rats. In Study B, serum values of glucose, triglycerides, and total cholesterol, and HDL, LDL and VLDL fractions were measured in 20 young rats and 10 adult rats. In Study C, pressure of the caudal artery was measured by plethysmography in 24 adult animals. Results: Total bilateral epididymectomy and orchiectomy reduced serum levels of testosterone regardless of the age of the animal, and apparently does not modify macro-or microscopically the morphology of the adrenal gland. Hypogonadism secondary to total bilateral epididymectomy and orchiectomy did not modify the serum values of glucose, total cholesterol, LDL and HDL fractions. However, orchiectomy reduced the blood levels of triglycerides and VLDL cholesterol when performed in young rats. Testosterone deficiency was related to smaller weight gain and increased arterial pressure. Conclusions: Orchiectomy reduces blood levels of triglycerides and VLDL cholesterol in young rats whereas in adult animals, arterial pressure increasing and smaller weight gain are verified. aspects of the adrenal gland in animals with hypogonadism secondary to total bilateral epididymectomy and orchiectomy. Material and Methods This project was carried out in observance of the recommendations of the International Norms for Protection of Animals and was approved by the Ethics Committee of Animal Experimentation. Male Wistar rats (Rattus norvegicus albinus) were used, both young (Y), 30-day-old, and mature adults (M), aged three to four months. The animals were allocated in appropriate cages with up to four rats per cage and kept under room temperature conditions with natural lighting, obeying a murine circadian cycle. They were observed daily and had no restrictions on supply of water and feed. The initial weight of the young rats varied between 55 and 80 g, and of the adults, it was between 250 and 350 g. The animals were randomly distributed into groups, according to the operative procedure performed. In Group 1, control, the rats were submitted to a simulated operation consisting of opening of the scrotum for exposure and manipulation of the testicles. And in Group 2, they were submitted to total bilateral epididymectomy and orchiectomy. All rats were anesthetized with an association of ketamine hydrochloride, with a dose of 50 mg/kg body weight and xylazine hydrochloride, with a dose of 5 mg/kg of body weight, intramuscularly in the gluteus region. During the entire period of anesthesia, heart and respiratory rate were observed, besides voluntary movement of The objective of the present paper was to study the modifications in lipid profile, blood glucose, arterial pressure, and morphological Case Report
doi:10.17352/ojbs.000001 fatcat:lxeqeyfcq5fxdjg6i2gvtpzmo4