Autophagy and apoptosis in starved and refed Neocaridina davidi (Crustacea, Malacostraca) midgut

A. Włodarczyk, S. Student, M. Rost-Roszkowska
2018 Canadian Journal of Zoology  
Canadian Journal of Zoology 2 1 2 Autophagy and apoptosis in starved and re-fed Neocaridina davidi 3 (Crustacea, Malacostraca) midgut 4 5 Włodarczyk A., Student S., Rost-Roszkowska M. * Abstract 8 Adult specimens of the freshwater shrimp Neocaridina davidi Bouvier, 1904 (Crustacea) were 9 starved for 7, 14 and 21 days. Specimens from first and second experimental group were 10 collected for the studies. The majority of animals starved for 21 days died. Additionally, some 11 specimens from each
more » ... pecimens from each group were re-fed for 4, 7, and 14 days. The epithelium of the midgut, 12 which is composed of the intestine and hepatopancreas, was analyzed. While the epithelium 13 of the intestine is formed by D-and R-cells, the epithelium of hepatopancreas has R-, B-and 14 F-cells. Autophagy and apoptosis in the midgut epithelium were analyzed using TEM and 15 immunohistochemical methods. These processes were only observed in the D-cells of the 16 intestine and the F-and B-cells of the hepatopancreas. Starvation led to a reduction in the 17 amount of reserve material in the B-cells. Although this process activated autophagy in both 18 regions of the midgut, the intestine and hepatopancreas, after re-feeding the level of 19 autophagy decreased. Starvation caused an increase in the apoptotic cells in both organs, 20 while the re-feeding caused a decrease in the number of apoptotic cells in both organs 21 analysed. Re-feeding after periods of starvation caused an accumulation of reserve material in 22 the hepatopancreas. 23 24 Page 2 of 38 Canadian Journal of Zoology 3 1
doi:10.1139/cjz-2018-0104 fatcat:jfdypaqaovgmdfeuzig43quyga