Evidenzbasierte Belastungseinstufung von Epilepsiemodellen [thesis]

Christina Möller
Evidence-based severity assessment in epilepsy models: Investigations in the amygdala kindling model in rats Epilepsies are one of the most common chronic neurological diseases in dogs and cats. Furthermore, around 65 million people worldwide are affected by epilepsies. In veterinary and human medicine, seizures are not medically controlled in about 30 % of patients. Therefore, it is essential to identify new target regions for antiepileptic drugs. Our understanding of the pathophysiological
more » ... thophysiological mechanisms of epilepsies and the discovery of the previous three generations of antiepileptic drugs is mainly based on knowledge gained through animal experimentation. In addition to the experimental animals in epilepsy models, experimental animals in many other research areas can exhibit seizure activity. To justify the use of experimental animals according to the legal regulations, a harm-benefit analysis weighing the potential benefits with the expected harm to the animal is required. Additionally, the burden of the animals affects the preclinical data quality. Therefore, evidence-based severity assessment schemes should be used to analyse the actual harm of the experimental animals. This should provide a basis for decisions by authorities and research groups. As part of a national research consortium focused on evidence-based severity assessment in different disease models, this study set out to explore the impact of repeated seizures and procedures on the well-being of the experimental animals. Comprehensive information was obtained about the overall pattern of neurobehavioral, biochemical and physiological alterations associated with the amygdala kindling model of epilepsy. For this purpose, various behavioural investigations were carried out, including the analysis of home cage behaviour, social interaction, burrowing behaviour, and behaviour associated with anxiety and depression. Furthermore, biochemical stress parameters in serum, faeces and hair samples were analysed. For the recording of home cage activity and hea [...]
doi:10.5282/edoc.22793 fatcat:7xaup2m6krcmnddcnv5uv4a4h4