A case of White Eye Syndrome in cultured whiteleg shrimp (litopenaeus vannamei) in concrete ponds

Ghajari, Meerbakhash, Yeganeh, Saaydi, Amrellahghajari@yahoo Com
2018 Downloaded from isfj.ir at 3:19 +0430 on Wednesday May 9th 2018 Downloaded from isfj.ir at 3:19 +0430 on Wednesday   unpublished
A month after movement of 1800 pieces of L. vannamei (average weight of 16g) from culture ponds in Heleh region to concrete ponds of Bandargah hatchery (Bushehr province) some shrimps showed symptoms such as decrease in growth rate, loss of appetite, dark pigmentation of cuticle and presence of black foci on it, redness of uropods and white, irregular and circular spots on the eye with 2-5mm diameters. For detection of etiological agent samples were taken for PCR, bacteriological and
more » ... ical and histo-pathological tests. Nested PCR test using IQ2000 kits for detection of major shrimp viral pathogens (IHHNV, WSSV, YHV and TSV) were conducted and all results were negative. In histo-pathological tests, lesions in eyes were liquefactive necrosis of cone cells and cone crystals with infiltration of hemocyte and formation of micro abscess in the diapteric region of eye. In bacteriological tests, a gram negative pleomorphic bacterium of the genus Aeromonas was isolated. However, it seems that this bacterium is the secondary agent and for detection of the primary agent more research is needed.