Examining go-or-grow using fluorescent cell-cycle indicators and cell cycle-inhibiting drugs [article]

Sean T Vittadello, Scott W McCue, Gency Gunasingh, Nikolas K Haass, Matthew J Simpson
2019 bioRxiv   pre-print
The go-or-grow hypothesis states that adherent cells undergo reversible phenotype switching between migratory and proliferative states, with cells in the migratory state being more motile than cells in the proliferative state. Here, we examine go-or-grow using melanoma cells with fluorescent cell-cycle indicators and cell cycle-inhibiting drugs. We analyse the experimental data using single-cell tracking to calculate mean diffusivities, and compare motility between cells in different cell-cycle
more » ... phases and in cell-cycle arrest. Unequivocally, our analysis does not support the go-or-grow hypothesis. We present clear evidence that cell motility is independent of the cell-cycle phase, and non-proliferative arrested cells have the same motility as cycling cells.
doi:10.1101/797142 fatcat:2cnpveljsnac5k2kflwwwvx6ba