Expression and DNA microarray analysis of a platelet activating factor-related molecule in severe pneumonia in mice due to influenza virus and bacterial co-infection

Masafumi Seki, Kosuke Kosai, Atsuko Hara, Yoshifumi Imamura, Shigeki Nakamura, Shintaro Kurihara, Koichi Izumikawa, Hiroshi Kakeya, Yoshihiro Yamamoto, Katsunori Yanagihara, Yoshitsugu Miyazaki, Hiroshi Mukae (+2 others)
2009 Japanese journal of infectious diseases (Print)  
Platelet-activating factor (PAF) is a critical mediator of severe inflammatory diseases such as pneumonia, and the PAF-receptor (PAFR) is known to be an anchor for Streptococcus pneumoniae attachment to lung epithelial cells. We conducted a DNA microarray analysis to detect critical factors that mediate fulminant pneumonia due to influenza virus and S. pneumoniae co-infection in mice. Among the factors detected, levels of PAF-acetyl hydrolase (PAF-AH), which functions as inactivated PAF, were
more » ... gnificantly increased, and PAFR was expressed in co-infected mouse lungs, as compared to the respective levels in mice infected with either S. pneumoniae or virus alone. Significantly elevated PAF-AH enzymatic activity was observed in the co-infected mouse lung, suggesting that co-infection activated PAF-related factors. These findings suggest that PAF and related molecules play important roles in fulminant pneumonia due to influenza virus infection, especially when severe bacterial pneumonia is complicated by co-infection with influenza virus.
pmid:19168951 fatcat:zc7frzciwbcljhqhvizg437sne