In Vitro Studies of Phospholipid Synthesis in Experimental Atherosclerosis

FRANK PARKER, JOHN W. ORMSBY, NORMAN F. PETERSON, GEORGE F. ODLAND, ROBERT H. WILLIAMS
1966 Circulation Research  
Using in vitro techniques and labeled linoleic acid and glucose, alterations in phospholipid synthesis in the aorta were correlated with electron microscopic studies at various intervals of time after feeding rabbits cholesterol. After 4 to 8 weeks of feeding, more phospholipid precursors were incorporated into die phospholipids of atherosclerotic blood vessels than of normal vessels. Concomitant with the metabolic alterations, the following ultrastructural changes occur. Smooth muscle cells of
more » ... oth muscle cells of the plaque (myo-intimal cells) evolve into highly vacuolated cells containing a profusion of cytoplasmic organelles. The increase in membranous organelles suggests that the increase in phospholipid synthesis may be the result of a cellular requirement for increased intracytoplasmic structural phospholipid. ADDITIONAL KEY WORDS atherosclerotic plaque smooth muscle cells phospholipid metabolism experimental atherosclerosis glucose metabolism intracellular organelles phospholipid synthesis electron microscopy linoleic acid blood vessel metabolism rabbit aorta
doi:10.1161/01.res.19.4.700 pmid:5917845 fatcat:axjmrn5d4rb7vhrmex275e3dte