Diversity of foliar endophytic fungi from the medicinal plant Sapindus saponaria L . and their localization by scanning electron microscopy

Adriana García, Sandro A Rhoden, Celso J Rubin Filho, Celso V Nakamura, João A Pamphile
2012 Biological Research  
Endophytic fungi inhabit vegetable tissues or organs, without causing them any harm. Endophytes can co-evolve with plant hosts and possess species-specifi c interactions. They can protect the plant from insect attacks and diseases, and are also able to produce substances of biotechnological interest. In folk medicine, the bark, roots and fruits of Sapindus saponaria is used to produce substances with anxiolytic, astringent, diuretic and expectorant properties, as well as tonics, blood
more » ... cs, blood depuratives and cough medicine. This study evaluated the diversity of endophytic fungi present in the leaves of S. saponaria L. and observed the colonization of host plants by endophytes, using light and scanning electron microscopy. We verifi ed that these fungi are found in intercellular and intracellular spaces. The genera of some isolates of S. saponaria were identifi ed mainly by sequencing of ITS region of rDNA and, when possible, also by their microscopic features, as follows: Cochliobolus, Alternaria, Curvularia, Phomopsis, Diaporthe and Phoma. Phylogenetic analysis showed the existence of genetic variability of the genera Phomopsis and Diaporthe and interspecifi c variation among the Curvularia, Alternaria and Phoma, belonging to family Pleosporaceae.
doi:10.4067/s0716-97602012000200006 pmid:23096358 fatcat:3w5zyc5c7vf3tcujzv3ctcezxa