Development and Validation of Analytical Method for The Lamivudine by Using Rp-Hplc Method

Rajender, K Subrahmanyam, Arabinda Patnaik
2015 IIII© International Journal of Medicine and Nanotechnology   unpublished
INTRODUCTION Analytical chemistry [1] involves separating, identifying, and determining the relative amounts of components in a sample matrix. Qualitative analysis reveals the chemical identity of the sample while Quantitative analysis establishes the International Journal of Medicine and Nanotechnology Access online at A selective, specific and sensitive stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of
more » ... e determination of Lamivudine. Reversed-phase chromatography was performed on Hitachi Elite Lachrome, equipped with UV detector using Develosil C18 column (150 mm × 4.6 mm, 5 μm). Mobile phase and flow rate selection was based on peak parameters (height, capacity, theoretical plates, tailing or symmetry factor), run time and resolution. The system with mobile phase containing 1%TEA: Acetonitrile (60:40% v/v) with 1 ml/min flow rate was quite robust. The optimum wavelength for detection was 237 nm at which better detector response for the drug was obtained. Chromatogram showed peak of Atenolol at retention time was 2.7 ± 0.057 min and Lamivudine at 4.28±0.048 min. The LOD & LOQ of Lamivudine was found to be 0.1 μg / ml . The percentage relative standard deviation in precision and accuracy studies was found to be less than 2%.