A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates

G.A. van der Marel, C.A.A. van Boeckel, G. Wille, J.H. van Boom
1982 Nucleic Acids Research  
A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with l-hydroxybenzotriazole. The approach will be examplified by the synthesis of pACGC using four different 5'-phosphotriester intermediates.
doi:10.1093/nar/10.7.2337 pmid:7088712 pmcid:PMC320613 fatcat:6brxcqknc5fpthcfxy6bq6ve5u