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A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with l-hydroxybenzotriazole. The approach will be examplified by the synthesis of pACGC using four different 5'-phosphotriester intermediates.doi:10.1093/nar/10.7.2337 pmid:7088712 pmcid:PMC320613 fatcat:6brxcqknc5fpthcfxy6bq6ve5u