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Viral Small RNA Cloning and Sequencing
At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects of virus biology in the context of host-pathogen interaction. However, the generation of high quality data is still an issue and the preparation of small RNAs libraries that accurately reflect the viral siRNAs in the sample remains a challenge. In this chapterdoi:10.1007/978-1-61779-037-9_6 pmid:21431681 fatcat:6l4ywldg5ja45pdm4vpz2xnjhu