Extended spectrum of Beta lactamases and metallo Beta lactamases producing resistant bacteria in nosocomial infections was increasing the morbidity and hospital expenditures. The source of infection is either nosocomial or community acquired or health care personnel. Comprehensive studies of source for hospital acquired infection will facilitate the control of hospital acquired infections.To emphasize the formation of hospital infection control committee (HICC) in tertiary care and teaching

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... itals, computerizing and preserving antibiotic resistant data in nosocomial infections and documenting the guidelines for antibiotic policy. Material and methods: Pus, urine, sputum and blood samples from outpatient and inpatients of MGM Hospitals of Dept. of Microbiology during January-2014 to April 2014 were processed by standard laboratory procedure as per CLSI. Anti microbial susceptibility testing was done by Kirby-Baure's disk diffusion method. ESBLs producing strains were detected by double disk synergy test after initial screening with 3 rd generation cephalosporins. MBL Producing organisms were detected by double disk synergy test with Imipenam and EDTA disks. Different swabs collected from NICU, AMC, Female surgical ward, Casuality, ICCU, ENT ward, Burns ward and Central Sterilization Department are processed by standard laboratory procedure as per CLSI guidelines. ESBLs producing strains were detected by double disk synergy test after initial screening with 3 rd generation cephalosporins. MBL Producing organisms were detected by double disk synergy test with Imipenam and EDTA disks. Results: Out of 217 blood culture positives 107 were ESBL (86) AND MBLS (21). Out of 240 sputum samples positive (72) were resistant bacterial strains ESBLS (49) and (23) were MBLS. Out of 185 pus culture samples (47) were resistant strains and ESBLS (35) and MBLS (12). Out of 88 swabs collected from various wards in MGM Hospital 30 were positive from resistant strains like 8 (ESBLS) and 3 MBLS and Vancomycin resistant staphylococci were 11 and remaining 8 were sensitive to routine antibiotics. Conclusion: The Study reveals that serious therapeutic and epidemiological spread of ESBLS and MBLS in Patients, Hospital environment hence the necessity of formation of Hospital Infection Control Committee (HICC) and to record the different patterns of antibiotic resistance in the tertiary hospitals and to upload the save in WHO NET will control the global emergence of MDR bacterial strains.